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quantitect primer assay gpbb  (Qiagen)


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    Qiagen quantitect primer assay gpbb
    G6Pase-β Is Expressed in Astrocytes from Rodents and Humans (A) Glucose transported into cells by glucose transporters (GLUT) is phosphorylated to G6P by HK. Glucose-exporting cells, like hepatocytes, use a G6P transporter (G6PT) to transport G6P into the ER, where it is dephosphorylated by G6Pase-α and then exported from the cell, possibly at ER-PM contact sites (top right). G6PT and G6Pase-α are viewed as adaptations that allow efficient glucose export. Astrocytes are proposed to provide neurons with a source of energy and neurotransmitter precursors by importing glucose at their perivascular endfeet, glycolytically metabolizing it, and then exporting neurotransmitter precursors and perhaps lactate at perisynaptic processes . Although the importance of the lactate shuttle has been questioned [ , , ], it is clear that astrocytes provide metabolic support to neurons. Our results suggest that G6PT and G6Pase-β allow the ER of astrocytes to serve as an intracellular highway moving glucose from perivascular endfeet to perisynaptic processes. (B and C) Confocal z stacks of rat brain cortical slices immunostained for G6Pase-β and GFAP showing their colocalization. Arrows in the overlays indicate the lumen of blood vessels surrounded by astrocytes. The overlay in (C) is additionally stained with isolectin B4 to identify capillaries (blue). (D) qPCR showing expression levels of mRNA (relative to GAPDH) of the indicated enzymes in human astrocytes: <t>GPBB</t> and GPMM are two isoforms of glycogen phosphorylase. Results show each independent determination (n = 4–8 isolates, derived from at least 4 different cultures) and the mean. (E) Immunoblots (30 μg protein/lane), typical of 3 similar blots from independent treatments, show expression of G6Pase-β and G6PT in human astrocytes.
    Quantitect Primer Assay Gpbb, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitect primer assay gpbb/product/Qiagen
    Average 90 stars, based on 1 article reviews
    quantitect primer assay gpbb - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Effective Glucose Uptake by Human Astrocytes Requires Its Sequestration in the Endoplasmic Reticulum by Glucose-6-Phosphatase-β"

    Article Title: Effective Glucose Uptake by Human Astrocytes Requires Its Sequestration in the Endoplasmic Reticulum by Glucose-6-Phosphatase-β

    Journal: Current Biology

    doi: 10.1016/j.cub.2018.08.060

    G6Pase-β Is Expressed in Astrocytes from Rodents and Humans (A) Glucose transported into cells by glucose transporters (GLUT) is phosphorylated to G6P by HK. Glucose-exporting cells, like hepatocytes, use a G6P transporter (G6PT) to transport G6P into the ER, where it is dephosphorylated by G6Pase-α and then exported from the cell, possibly at ER-PM contact sites (top right). G6PT and G6Pase-α are viewed as adaptations that allow efficient glucose export. Astrocytes are proposed to provide neurons with a source of energy and neurotransmitter precursors by importing glucose at their perivascular endfeet, glycolytically metabolizing it, and then exporting neurotransmitter precursors and perhaps lactate at perisynaptic processes . Although the importance of the lactate shuttle has been questioned [ , , ], it is clear that astrocytes provide metabolic support to neurons. Our results suggest that G6PT and G6Pase-β allow the ER of astrocytes to serve as an intracellular highway moving glucose from perivascular endfeet to perisynaptic processes. (B and C) Confocal z stacks of rat brain cortical slices immunostained for G6Pase-β and GFAP showing their colocalization. Arrows in the overlays indicate the lumen of blood vessels surrounded by astrocytes. The overlay in (C) is additionally stained with isolectin B4 to identify capillaries (blue). (D) qPCR showing expression levels of mRNA (relative to GAPDH) of the indicated enzymes in human astrocytes: GPBB and GPMM are two isoforms of glycogen phosphorylase. Results show each independent determination (n = 4–8 isolates, derived from at least 4 different cultures) and the mean. (E) Immunoblots (30 μg protein/lane), typical of 3 similar blots from independent treatments, show expression of G6Pase-β and G6PT in human astrocytes.
    Figure Legend Snippet: G6Pase-β Is Expressed in Astrocytes from Rodents and Humans (A) Glucose transported into cells by glucose transporters (GLUT) is phosphorylated to G6P by HK. Glucose-exporting cells, like hepatocytes, use a G6P transporter (G6PT) to transport G6P into the ER, where it is dephosphorylated by G6Pase-α and then exported from the cell, possibly at ER-PM contact sites (top right). G6PT and G6Pase-α are viewed as adaptations that allow efficient glucose export. Astrocytes are proposed to provide neurons with a source of energy and neurotransmitter precursors by importing glucose at their perivascular endfeet, glycolytically metabolizing it, and then exporting neurotransmitter precursors and perhaps lactate at perisynaptic processes . Although the importance of the lactate shuttle has been questioned [ , , ], it is clear that astrocytes provide metabolic support to neurons. Our results suggest that G6PT and G6Pase-β allow the ER of astrocytes to serve as an intracellular highway moving glucose from perivascular endfeet to perisynaptic processes. (B and C) Confocal z stacks of rat brain cortical slices immunostained for G6Pase-β and GFAP showing their colocalization. Arrows in the overlays indicate the lumen of blood vessels surrounded by astrocytes. The overlay in (C) is additionally stained with isolectin B4 to identify capillaries (blue). (D) qPCR showing expression levels of mRNA (relative to GAPDH) of the indicated enzymes in human astrocytes: GPBB and GPMM are two isoforms of glycogen phosphorylase. Results show each independent determination (n = 4–8 isolates, derived from at least 4 different cultures) and the mean. (E) Immunoblots (30 μg protein/lane), typical of 3 similar blots from independent treatments, show expression of G6Pase-β and G6PT in human astrocytes.

    Techniques Used: Staining, Expressing, Derivative Assay, Western Blot


    Figure Legend Snippet:

    Techniques Used: Immunocytochemistry, Western Blot, Bacteria, Virus, Plasmid Preparation, Recombinant, ATP Assay, shRNA, Software



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    quantitect primer assay gpbb  (Qiagen)
    90
    Qiagen quantitect primer assay gpbb
    G6Pase-β Is Expressed in Astrocytes from Rodents and Humans (A) Glucose transported into cells by glucose transporters (GLUT) is phosphorylated to G6P by HK. Glucose-exporting cells, like hepatocytes, use a G6P transporter (G6PT) to transport G6P into the ER, where it is dephosphorylated by G6Pase-α and then exported from the cell, possibly at ER-PM contact sites (top right). G6PT and G6Pase-α are viewed as adaptations that allow efficient glucose export. Astrocytes are proposed to provide neurons with a source of energy and neurotransmitter precursors by importing glucose at their perivascular endfeet, glycolytically metabolizing it, and then exporting neurotransmitter precursors and perhaps lactate at perisynaptic processes . Although the importance of the lactate shuttle has been questioned [ , , ], it is clear that astrocytes provide metabolic support to neurons. Our results suggest that G6PT and G6Pase-β allow the ER of astrocytes to serve as an intracellular highway moving glucose from perivascular endfeet to perisynaptic processes. (B and C) Confocal z stacks of rat brain cortical slices immunostained for G6Pase-β and GFAP showing their colocalization. Arrows in the overlays indicate the lumen of blood vessels surrounded by astrocytes. The overlay in (C) is additionally stained with isolectin B4 to identify capillaries (blue). (D) qPCR showing expression levels of mRNA (relative to GAPDH) of the indicated enzymes in human astrocytes: <t>GPBB</t> and GPMM are two isoforms of glycogen phosphorylase. Results show each independent determination (n = 4–8 isolates, derived from at least 4 different cultures) and the mean. (E) Immunoblots (30 μg protein/lane), typical of 3 similar blots from independent treatments, show expression of G6Pase-β and G6PT in human astrocytes.
    Quantitect Primer Assay Gpbb, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitect primer assay gpbb/product/Qiagen
    Average 90 stars, based on 1 article reviews
    quantitect primer assay gpbb - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

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    G6Pase-β Is Expressed in Astrocytes from Rodents and Humans (A) Glucose transported into cells by glucose transporters (GLUT) is phosphorylated to G6P by HK. Glucose-exporting cells, like hepatocytes, use a G6P transporter (G6PT) to transport G6P into the ER, where it is dephosphorylated by G6Pase-α and then exported from the cell, possibly at ER-PM contact sites (top right). G6PT and G6Pase-α are viewed as adaptations that allow efficient glucose export. Astrocytes are proposed to provide neurons with a source of energy and neurotransmitter precursors by importing glucose at their perivascular endfeet, glycolytically metabolizing it, and then exporting neurotransmitter precursors and perhaps lactate at perisynaptic processes . Although the importance of the lactate shuttle has been questioned [ , , ], it is clear that astrocytes provide metabolic support to neurons. Our results suggest that G6PT and G6Pase-β allow the ER of astrocytes to serve as an intracellular highway moving glucose from perivascular endfeet to perisynaptic processes. (B and C) Confocal z stacks of rat brain cortical slices immunostained for G6Pase-β and GFAP showing their colocalization. Arrows in the overlays indicate the lumen of blood vessels surrounded by astrocytes. The overlay in (C) is additionally stained with isolectin B4 to identify capillaries (blue). (D) qPCR showing expression levels of mRNA (relative to GAPDH) of the indicated enzymes in human astrocytes: GPBB and GPMM are two isoforms of glycogen phosphorylase. Results show each independent determination (n = 4–8 isolates, derived from at least 4 different cultures) and the mean. (E) Immunoblots (30 μg protein/lane), typical of 3 similar blots from independent treatments, show expression of G6Pase-β and G6PT in human astrocytes.

    Journal: Current Biology

    Article Title: Effective Glucose Uptake by Human Astrocytes Requires Its Sequestration in the Endoplasmic Reticulum by Glucose-6-Phosphatase-β

    doi: 10.1016/j.cub.2018.08.060

    Figure Lengend Snippet: G6Pase-β Is Expressed in Astrocytes from Rodents and Humans (A) Glucose transported into cells by glucose transporters (GLUT) is phosphorylated to G6P by HK. Glucose-exporting cells, like hepatocytes, use a G6P transporter (G6PT) to transport G6P into the ER, where it is dephosphorylated by G6Pase-α and then exported from the cell, possibly at ER-PM contact sites (top right). G6PT and G6Pase-α are viewed as adaptations that allow efficient glucose export. Astrocytes are proposed to provide neurons with a source of energy and neurotransmitter precursors by importing glucose at their perivascular endfeet, glycolytically metabolizing it, and then exporting neurotransmitter precursors and perhaps lactate at perisynaptic processes . Although the importance of the lactate shuttle has been questioned [ , , ], it is clear that astrocytes provide metabolic support to neurons. Our results suggest that G6PT and G6Pase-β allow the ER of astrocytes to serve as an intracellular highway moving glucose from perivascular endfeet to perisynaptic processes. (B and C) Confocal z stacks of rat brain cortical slices immunostained for G6Pase-β and GFAP showing their colocalization. Arrows in the overlays indicate the lumen of blood vessels surrounded by astrocytes. The overlay in (C) is additionally stained with isolectin B4 to identify capillaries (blue). (D) qPCR showing expression levels of mRNA (relative to GAPDH) of the indicated enzymes in human astrocytes: GPBB and GPMM are two isoforms of glycogen phosphorylase. Results show each independent determination (n = 4–8 isolates, derived from at least 4 different cultures) and the mean. (E) Immunoblots (30 μg protein/lane), typical of 3 similar blots from independent treatments, show expression of G6Pase-β and G6PT in human astrocytes.

    Article Snippet: Quantitect primer assay: GPBB , QIAGEN , Cat#Hs_PYGB_1_SG.

    Techniques: Staining, Expressing, Derivative Assay, Western Blot

    Journal: Current Biology

    Article Title: Effective Glucose Uptake by Human Astrocytes Requires Its Sequestration in the Endoplasmic Reticulum by Glucose-6-Phosphatase-β

    doi: 10.1016/j.cub.2018.08.060

    Figure Lengend Snippet:

    Article Snippet: Quantitect primer assay: GPBB , QIAGEN , Cat#Hs_PYGB_1_SG.

    Techniques: Immunocytochemistry, Western Blot, Bacteria, Virus, Plasmid Preparation, Recombinant, ATP Assay, shRNA, Software